Fig. 6
From: Rice Basic Helix-Loop-Helix 079 (OsbHLH079) Delays Leaf Senescence by Attenuating ABA Signaling
Expression analysis of chlorophyll degradation genes during natural senescence of flag leaves. A-G Relative mRNA levels of OsNYC1 (A), OsNOL (B), OsHCAR (C), OsSGR (D), OsNYC3 (E), OsPAO (F), and OsRCCR1 (G) were compared among WT, osbhlh079-D, OsbHLH079-OE, osbhlh079-1, and osbhlh079-2. Total RNA samples from Fig. 5 were subjected to RT-qPCR analysis, using GAPDH as a reference for normalization. Data are presented as mean ± SD of four independent biological samples, each with approximately five flag leaves per sample. Asterisks on the bars indicate statistically significant differences compared to WT transcript levels (Student’s t-test; *P < 0.05, **P < 0.01, and ***P < 0.001). These experiments were performed twice with similar results. DAH, days after heading; NS, not significant