Fig. 5
From: Rice Basic Helix-Loop-Helix 079 (OsbHLH079) Delays Leaf Senescence by Attenuating ABA Signaling
Expression profiles of senescence-associated genes in naturally senescing flag leaves. A-G Relative transcript levels of Osl2 (A), Osl20 (B), Osl55 (C), Osl57 (D), Osl85 (E), Osh69 (F), and OsSAG12-2 (G) in WT, osbhlh079-D, OsbHLH079-OE, osbhlh079-1, and osbhlh079-2. The middle parts of the flag leaves were harvested from the rice plants grown under natural long-day conditions in the paddy field at the indicated DAH and were used for RT-qPCR analysis. The expression levels of each gene were normalized to that of GAPDH, an internal control. The values presented are the means of four biological replicates, with each replicate consisting of five independent flag leaves, and the error bars indicate the standard deviations. Significance of differences between means was analyzed by the two-tailed Student’s t-test (*P < 0.05, **P < 0.01, and ***P < 0.001). These analyses were performed twice independently with similar results. DAH, days after heading; NS, not significant