Fig. 3

Localization analysis of PRX102. Shown are: A–H, Subcellular localization of PRX102 in transgenic plants carrying the pPRX102::PRX102–GFP cassette. Photos were taken at the transition zone (A and B), the elongation zone (C and D), and the maturation zone of the root (E–H). GFP images in root hairs at fast growing stage (E and F) and growth termination stage (G and H). Arrows indicate accumulated GFP signal at trichoblasts root hairs. I and J, Localization of PRX102 after plasmolysis using 500 mM mannitol. Fluorescent images (A, C, E, G, and I) and blight-field merged images (B, D, F, H, and J). RFP–PRX102 (K and N), ER marker (L), Golgi marker (O) and merged image (M and P) in leaf epidermal cells from Nicotiana benthamiana. Large yellow boxes at bottom left corner of K–P are the magnification of small yellow boxes in each image. White and red arrowheads mark overlapping signals and non-overlapping signals, respectively. Also shown are cells expressing 35S::GFP as control (Q). Scale bars = 20 μm (A–H), 10 μm (I–J), and 50 μm (K–Q)