Fig. 2

Analysis of metabolite abundance changes in two directly seeded rice varieties at different time points. a Overview of the experimental design. Seeds of two directly seeded rice varieties were imbibed in liquid medium under aerobic or submerged conditions. Three biological replicates for analysis of metabolism were obtained at 0, 2, 3, and 4 DAI (day after imbibition) under both growth conditions (the 0-d time point was common to both treatments). Additionally, after 3 days of growth, samples were switched, where aerobically grown samples were switched to anaerobic conditions, and samples were taken 1 d after switching. Submerged germinated seeds were switched to aerobic conditions, and samples were taken at the same time points. This yielded six datasets in which metabolic changes in metabolite abundance were analyzed. Time points for metabolite profiling analysis are indicated in gray. Three additional time points were included. Red indicates aerobic conditions, and blue indicates submerged conditions. b Summary of the changes in metabolite abundance during the experiments outlined in A. The number of metabolites is indicated on the ordinate. The time points compared are indicated on the abscissa