Integrating Association Mapping, Linkage Mapping, Fine Mapping with RNA Seq Conferring Seedling Vigor Improvement for Successful Crop Establishment in Deep Sown Direct-Seeded Rice

Sandhu, Nitika; Ankush, Ade Pooja; Singh, Jasneet; Raigar, Om Prakash; Bains, Sutej; Jindal, Taveena; Singh, Mohini Prabha; Sethi, Mehak; Pruthi, Gomsie; Augustine, Gaurav; Verma, Vikas Kumar; Goyal, Shivani; Kumar, Aman; Panwar, Harsh; Sihag, Manvesh Kumar; Kaur, Rupinder; Kurup, Smita; Kumar, Arvind

doi:10.1186/s12284-023-00665-w

Rice

Table 1 Detailed information on analysis of variance (ANOVA), mean, range and heritability of the traits measured across years in different growing environments in both F_3:4:5 and BC₂F_2:3 mapping populations in background of PR126

From: Integrating Association Mapping, Linkage Mapping, Fine Mapping with RNA Seq Conferring Seedling Vigor Improvement for Successful Crop Establishment in Deep Sown Direct-Seeded Rice

Environment	Generation	Trait	Year	PR126	R146	PR126/R146		F value	p value	Heritability
Environment	Generation	Trait	Year	Mean + SE	Mean + SE	Mean + SE	Range	F value	p value	Heritability
Screenhouse	F₃:F₄	%germination_4 cm	2021	0	100 ± 4.2	10.11 ± 3.10	0–100	2.166	***	0.71
	F₄:F₅		2022	16.67 ± 3.31	90.30 ± 5.8	39.98 ± 8.21	0–100	2.9267	***	0.64
	F₃:F₄	%germination_10 cm	2021	0	96.97 ± 1.51	19.15 ± 4.45	0–100	35.7209	***	0.82
	F₄:F₅		2022	0	92.21 ± 2.19	17.64 ± 3.44	0–100	1.4026	*	0.77
	F₃:F₄	Mesocotyl length_4 cm	2021	0	5.64 ± 0.027	4.40 ± 0.12	0–6	3.342	**	0.62
	F₄:F₅		2022	0	4.21 ± 0.65	4.00 ± 1.52	0–6	1.6436	**	0.55
	F₃:F₄	Mesocotyl length_10 cm	2021	0	8.21 ± 0.23	2.75 ± 0.75	0–10	29.948	***	0.76
	F₄:F₅		2022	0	8.17 ± 0.94	3.52 ± 1.52	0–10	2.0515	**	0.69
	F₃:F₄	Root length_4 cm	2021	0.00	4.80 ± 0.1	4.43 ± 0.44	0–15.6	108.059	***	0.69
	F₄:F₅		2022	0.27 ± 0.08	5.27 ± 0.79	4.61 ± 1.06	0–16.2	2.1414	***	0.53
	F₃:F₄	Root length_10 cm	2021	0.00	8.60 ± 0.1	1.650 ± 0.23	0–18	76.9385	***	0.55
	F₄:F₅		2022	0.00	7.30 ± 0.75	2.28 ± 1.12	0–13.7	1.4029	*	0.49
	F₃:F₄	Shoot length_4 cm	2021	0.00	22.87 ± 0.35	10.23 ± 1.66	0–26.35	68.46	***	0.72
	F₄:F₅		2022	0.00	23.84 ± 2.36	10.69 ± 1.54	0–33.17	2.0776	**	0.62
	F₃:F₄	Shoot length_10 cm	2021	0.00	24.07 ± 1.15	15.28 ± 0.70	0–36.6	694.327	***	0.77
	F₄:F₅		2022	0.00	27.54 ± 2.80	10.42 ± 0.89	0–33.0	2.0179	**	0.77
Field	F₃:F₄	Days to 50% flowering	2021	80 ± 0.91	66.09 ± 0.91	69.36 ± 4.28	53–89	4.9315	***	0.76
	F₄:F₅		2022	77 ± 1.19	65.64 ± 1.19	67.93 ± 5.54	55–89	3.006	***	0.67
	F₃:F₄	Plant height	2021	82 ± 3.49	104.10 ± 4.13	113.09 ± 10.16	64–161	2.652	***	0.7
	F₄:F₅		2022	75 ± 3.88	91.45 ± 3.21	109.72 ± 10.51	60–155	2.351	***	0.66
	F₃:F₄	Panicles per plant	2021	8 ± 0.45	9.91 ± 0.62	9.45 ± 2.00	4–32	4.5217	***	0.61
	F₄:F₅		2022	6 ± 0.44	6.73 ± 0.48	5.92 ± 2.01	1–21	2.1306	***	0.67
	F₃:F₄	Panicle length	2021	24 ± 0.63	18.91 ± 0.54	21.86 ± 2.95	16–30	1.2973	*	0.72
	F₄:F₅		2022	19 ± 0.86	16.55 ± 0.89	20.99 ± 3.57	13–29	1.0498	*	0.65
	F₃:F₄	1000-grain weight	2021	16.23 ± 0.88	17.46 ± 0.76	13.78 ± 0.83	5.3–28.83	3.336	**	0.62
	F₄:F₅		2022	12.32 ± 0.69	13.22 ± 0.55	10.33 ± 0.49	2.6–23.58	3.016	***	0.61
	F₃:F₄	Filled grains/panicle	2021	52.0 ± 2.2	45.0 ± 1.6	38.0 ± 2.5	25.0–64.0	3.121	***	0.58
	F₄:F₅		2022	45.0 ± 2.5	40.0 ± 1.9	32.0 ± 2.8	22.0–58.0	3.332	**	0.55
	F₃:F₄	Grain yield	2021	1006 ± 87.88	1031.82 ± 92.34	1439 ± 268	217–3183	5.8333	***	0.82
	F₄:F₅		2022	509 ± 60.92	568.18 ± 54.46	717 ± 181	176–2812	4.989	***	0.70
Screenhouse	BC₂F_2:3	% of germination_4 cm	2022	16.67 ± 3.88	100 ± 3.6	47.97 ± 5.56	8.33–91.67	2.251	**	0.74
	BC₂F_2:3	% of germination_10 cm	2022	0	98.86 ± 1.8	37.03 ± 4.45	0.0–91.67	1.475	***	0.61
	BC₂F_2:3	Mesocotyl length_4 cm	2022	0.88 ± 0.06	4.56 ± 1.5	5.14 ± 1.15	0.0–6.0	3.344	**	0.66
	BC₂F_2:3	Mesocotyl length_10 cm	2022	0	8.8 ± 1.32	6.32 ± 0.99	0.0–9.43	2.853	***	0.69
	BC₂F_2:3	Root length_4 cm	2022	0.85 ± 0.9	5.5 ± 1.03	3.9 ± 0.92	0.0–11.36	2.14	***	0.62
	BC₂F_2:3	Root length_10 cm	2022	0	7.7 ± 1.1	2.6 ± 0.87	10.0–10.9	10.248	***	0.57
	BC₂F_2:3	Shoot length_4 cm	2022	0	20.02 ± 1.15	12.80 ± 1.51	0.0–22.27	2.575	***	0.79
	BC₂F_2:3	Shoot length_10 cm	2022	0	22.45 ± 1.03	11.96 ± 1.72	0.0–29.0	3.227	**	0.76

%germination: percentage germination, days to 50% flowering (days), plant height (cm), panicle length (cm), grain yield (kg/ha), root length (cm), shoot length (cm), mesocotyl length (cm), 1000-grain weight (gm), 4 cm: when the seed sown at 4 cm below soil surface (control), 10 cm: when the seed sown at 10 cm below soil surface (treatment)

Back to article page