Fig. 4

(a) The pictorial representation of the 8 KASP assays conducted on the 339 BC₂F_2:3 plants including parents. Blue color indicates the donor allele, red color indicates the recipient allele and green color indicates the heterozygotes. (b) The schematic representation of the library size for 106 samples used to generate RNA-seq data (c) The volcano plot showing differentially expressed genes in group I (d) The volcano plot showing differentially expressed genes in group II (e) The pictorial representation of the measurement of mesocotyl length using vernier calliper, phenotypic screening of the backcross population under control (4 cm) and deep sown (10 cm) conditions (f) The performance of selected promising breeding in comparison to recipient parent (PR126) and donor parent (IRGC 128442) lines in terms of mesocotyl elongation (ML), root length (RL) and shoot length (SL) under control (4 cm) and deep sown (10 cm) conditions (g) The performance of selected promising breeding in comparison to recipient parent (PR126) and donor parent (IRGC 128442) lines in terms of %germination under control (4 cm) and deep sown (10 cm) conditions (h) The allelic constitution of the selected breeding lines, recipient parent (PR126) and donor parent (IRGC 128442) in the fine mapped region on chromosome 7. D: donor parent allele, R: recipient parent allele