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Rice
Fig. 3 | Rice

Fig. 3

From: Simultaneous Enhancement of iron Deficiency Tolerance and Iron Accumulation in Rice by Combining the Knockdown of OsHRZ Ubiquitin Ligases with the Introduction of Engineered Ferric-chelate Reductase

Fig. 3

Features of the HRZ2i + Refre lines under pot cultivation in calcareous soil with non-submerged conditions. A The Soil Plant Analysis Development (SPAD) value of the newest leaves. B Shoot height. C Representative plants on day 29 after transplanting. Scale bar = 10 cm. D Panicle and straw dry weights after seed maturation. E Fe concentrations in brown seeds and straw following seed maturation. The following lines were cultivated: NT, non-transformants; HRZ2i + Refre, T1 lines of 1, 5, 7, and 9, and T2 line of 2–2. DW: dry weight. For A and B, the means ± standard error (SE) are shown (n = 4). Asterisks indicate significant differences compared to the NT at each time point (two-sample Student’s t-test; *P < 0.05; **P < 0.01). For D and E, the means ± SD are shown (n = 4 for all groups in D and HRZ2i + Refre lines 2–2, 5, 7, and 9 for seed Fe; n = 2 for NT for seed Fe; and n = 3 for HRZ2i + Refre line 1 for seed Fe and all lines for straw Fe). Asterisks indicate significant differences compared to the NT (two-sample Student’s t-test; *P < 0.05; **P < 0.01)

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