Fig. 5
OsIMα1a enhanced OsWRKY62.1 acting as a negative regulator of resistance against rice blast pathogen. a The 3-month-old transgenic and wild-type ZH17 plants were inoculated with a virulent strain of M. oryzae SZ by infiltration. Symptoms were photographed 10 days after the challenge. Bar = 2 cm. b Lesion length was measured from the inoculated leaves. Significant differences (Duncan's multiple range test; α = 0.05) compared with the wild-type plants are listed in the figure. c Change of OsWRKY62.1-GFP localization through mutation of NES-like sequence in OsWRKY62.1. The key amino acids in NES-like sequence of OsWRKY62.1 were mutated to A residues (W62.1nes4A). The plasmids of 35S::W62.1nes4A-GFP and 35S::W62.1-GFP, in combination with 35S::dsREDNLS were introduced into the leaf cells of N. benthamiana, respectively. Confocal images were taken 72 h after the agroinfiltration. From left panel to right: GFP images (GFP), dsRED images (RED), and combined YFP and RED in the bright field (Merged). Bar = 20 µm