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Rice
Fig. 3 | Rice

Fig. 3

From: SDG712, a Putative H3K9-Specific Methyltransferase Encoding Gene, Delays Flowering through Repressing the Expression of Florigen Genes in Rice

Fig. 3

Identification and phenotypic analysis of sdg712. A, Schematic of Tos17 insertion site in sdg712 mutant. Open boxes denote 5’UTR and 3’UTR; filled boxes denote exons. Lines are introns, and arrows denote primers of detection. B, Identification of homozygous sdg712 mutant using primers pNG0566F, pNG0566R and pTail3 (indicated by arrows in A). Primers pNG0566F and pNG0566R are located in the fourth exon of sdg712 and pTail3 is located in the left border of Tos17. M, DNA ladder. WT is Nipponbare. C, The expression of SDG712 in sdg712 mutant and WT Nipponbare. OsActin1 was used as internal control. D, The phenotype of sdg712 under natural long-day condition. Bar = 20 cm. WT is Nipponbare. E, Heading-date of sdg712 under natural long-day (NLD) and natural short-day (NSD) conditions. Values are shown as means ± SD. N ≥ 30 individual plants. WT is Nipponbare. Asterisks indicate statistically significant differences of t-test (**, P < 0.01)

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