Fig. 9

Effect of obgc1 mutation on the accumulation of plastid precursor rRNA. A Abundance of rRNAs. One microgram of total RNAs from 10-day-old wild-type (Dongjin) and obgc1-d1 seedlings was separated on a denaturing gel, and then stained with EtBr (ethidium bromide). “23S*” (approximate 1.2 kb) is the breakdown product of chloroplast 23S rRNA. B obgc1-d mutation causes the accumulation of precursor 23S or 16S rRNAs. Northern blot analyses of plastid rRNAs were performed using total RNAs (1x, 1 μg; 0.1x, 0.1 μg; 10x, 10 μg). Blots were probed with gene-specific sequences for 16S/23S rRNAs. Cytosolic 17S rRNA was used as a loading control