Fig. 2
From: PREMATURE SENESCENCE LEAF 50 Promotes Heat Stress Tolerance in Rice (Oryza sativa L.)
Effects of heat stress on psl50 and WT at the seedling stage. a Phenotypes of WT and psl50 seedlings under heat stress. Seedlings were hydroponically (upper row) or soil grown (lower row) at 26 °C for 12 d and then treated at 45 °C for 50 h or 40 h followed a recovery at 26 °C. Scale bars = 5 cm. b DAB staining for H2O2 accumulation detection and trypan blue staining for cell death detection in the top 2nd leaves of hydroponical seedlings before and after heat treatment. c Survival rate of hydroponic WT and psl50 seedlings shown in a. d-f H2O2 content, ion leakage rate and MDA content in hydroponic WT and psl50 seedlings before and after heat treatment. Data are means ± SD (n = 3). Asterisks indicate significant difference by Student’s t test (**P < 0.01 and *P < 0.05)