Fig. 3

APIP12 is required for the basal resistance to M. oryzae in rice. a Transcriptional pattern of APIP12 in both compatible (NPB against KJ201) and incompatible (NPB-Piz-t against KJ201) interactions. The abundance of transcripts of APIP12 is quantified at 0, 12, 24, 36, 48 and 72 h post inoculation (HPI) by semi-quantitative RT-PCR. Mock infection without pathogen is used as the control. Actin is used as an internal control gene. b Disease symptom of APIP12 knockout mutant (APIP12-KO) and wild type (ZH11) after inoculation with M. oryzae isolate GUY11. Photograph of two representative leaves is taken 7 days after spray inoculation. c Quantification of lesion density in APIP12-KO and ZH11. The lesions at type 3 or above were counted in over 40 diseased leaves and the average lesion number was then calculated. Asterisks indicate statistically significant differences between APIP12-KO line and ZH11 (Student’s t-test, **P < 0.01). d Disease lesions in APIP12-KO and ZH11 in punch inoculation with M. oryzae isolate GUY11. Photograph of two representative leaves is taken 10 days after inoculation. e Quantitative analysis of disease lesions in APIP12-KO and ZH11 in punch inoculation. Error bars indicate standard deviation (SD) obtained from eight biological replicates (n = 8) and asterisks indicate statistically significant differences compared with ZH11 (Student’s t-test, ^** P < 0.01)