Fig. 2

The physical binding of APIP12 with AvrPiz-t and APIP6. a The protein-protein interaction between AvrPiz-t and APIP12 in yeast two-hybrid assay. Four APIP12 fragments (F, N, M, C) were used for the test of the interaction with AvrPiz-t. The interactions between the tested proteins were assayed by monitoring yeast colonies growth on selective medium DOB-Leu-Trp-His to detect the activation of the His reporter gene. The LacZ reporter gene activity was detected for blue color of yeast colonies on a filter paper containing X-gal. b The protein-protein interaction between AvrPiz-t and APIP12 in GST pull-down assay. Different APIP12 fragments (F, N, M) each are fused with the GST tag and AvrPiz-t is fused with the MBP tag. The combination of GST- and MBP-fusion proteins are tested for the binding by immunoblotted using anti-GST (top panel) or anti-MBP (bottom panel) before (5% input) and after pull-down (Pulldown). GST was used as a negative control. The band corresponding to the expected size of the fusion protein is indicated in an arrow deduced based on the size of the protein ladder in the right. c The protein-protein interaction between APIP12 and APIP6 in yeast two-hybrid assay. d The protein-protein interaction between APIP12 and APIP6 in GST pull-down assay