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Fig. 9 | Rice

Fig. 9

From: OsWRKY80-OsWRKY4 Module as a Positive Regulatory Circuit in Rice Resistance Against Rhizoctonia solani

Fig. 9

Transient assay for the interaction between OsWRKY80 and the promoter of OsWRKY4 in tobacco. a Diagram of the promoter of WRKY4 showing W-box or W-box like elements (bold vertical lines) in different regions. b Transient expression assay in tobacco. Full-length (P1) and 5’-deleted (P2, P3 and P4) promoters of OsWRKY4 were constructed into the report vector, and OsWRKY80 was cloned into the effect vector, respectively. LUC, Firefly luciferase activity; REN, Renilla luciferase activity (used as the control). Data are represented as mean values ± SE for three replicates. Asterisks indicate a significant difference (** P < 0.01) between the co-infiltration with reporter and effector vectors and the infiltration with reporter vectors alone according to Duncan’s multiple range test

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