Fig. 3

Transcriptional activation activity of OsWRKY80 in yeast cells. The full encoding sequence and deletion derivatives of OsWRKY80 were fused in frame to the GAL4 binding domain (BD) in pBDKT7 (pBD) to generate various vectors for yeast transformation. a The constructed vectors were transformed into yeast AH109 strain, and grew on the selective medium at 30°C for 3 d. Yeast cells carrying different constructs grew on SD-Trp medium (left panel), or SD-Trp-Ade-His (right panel). Middle panel, schematic distribution of yeast cells carrying different vectors. b Assay for a-galactosidase activity. Empty vector pBD and pBD-WRKY4 (Wang et al, 2015) were used as negative and positive control, respectively. The enzymatic activity of cells carrying pBD-WRKY80 was set as 100%. Data are represented as mean values ± SE for three replicates. *, ** indicate a significant difference at P < 0.05 and 0.01, respectively, between the transformant for pBD-WRKY80 and other vectors according to Duncan’s multiple range test. Grey, black rectangles represent BD in pGBKT7 and WRKY domains, respectively. The numbers in each construct are the start and end positions of translation product of OsWRKY80