QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice

Rice

Table 1 Comparison of 13 traits between two parents and among three groups

Traits^a)		Mean			Mean ± S.D			ANOVA^f)
Traits^a)		Hwaseong	O. rufipogon	Diff.^b)	96 lines^c)	83 lines^d)	13 lines^e)	Year (Y)	Genotype (G)	Y × G
Days to heading		108	109	ns	105 ± 6b ^g)	107 ± 3b	94 ± 4a
Rice quality	HR (%)	85	26	**	82.7 ± 13.4b	85.7 ± 10.2b	63.8 ± 15.9a	22.1**	325.2**	3.3**
	CR (%)	6.2	34.7	**	10.0 ± 11.7a	7.9 ± 8.7a	23.4 ± 17.8b	31.1**	664.2**	4.8**
	PC (%)	6.0	7.2	**	6.3 ± 0.6a	6.2 ± 0.5a	7.1 ± 0.6b	5.9**	32.1**	4.1**
	AC (%)	18.8	25.3	**	18.5 ± 0.7b	18.6 ± 0.5b	17.5 ± 0.7a	6.3**	229.1**	1.9**
	G C R	69.2	44.8	**	68 ± 7b	69 ± 5b	59 ± 10a	7.5**	152.1**	1.6**
Starch viscosity	PST (°C)	75.4	79.3	**	77.4 ± 5.4a	76.9 ± 5.5a	80.2 ± 3.6b	1.7**	186.2**	1.1^ns
	PKV (RVU)	244.5	177.9	**	234.4 ± 34.9a	230.9 ± 29.3a	255.9 ± 55.3b	6.9**	765.1**	1.1 ^ns
	HPV (RVU)	133.9	131.1	ns	138.5 ± 19.6	137.5 ± 18.4	145.0 ± 25.4	10.0**	755.2**	1.0 ^ns
	BDV (RVU)	88.6	57.8	**	95.7 ± 22.8a	93.4 ± 19.5a	110.9 ± 34.8b	3.9**	1013.0**	0.7 ^ns
	CPV (RVU)	236.3	185.8	**	243.7 ± 30.1a	242.7 ± 28.9a	250.6 ± 36.5a	5.2**	11.0**	0.6 ^ns
	SBV (RVU)	−18.22	7.9	**	9.5 ± 22.1b	11.7 ± 17.9b	−5.4 ± 36.9a	4.1**	97.1**	0.6 ^ns
	CSV (RVU)	102.4	54.7	**	105.0 ± 14.5a	105.0 ± 13.8a	104.6 ± 18.4a	2.8**	1208.0**	0.6 ^ns

a) HR Head rice ratio, CR Chalky rice ratio, PC Protein content, AC Amylose content, GCR Glossiness of cooked rice, PST pasting temperature, PKV peak viscosity, HPV hot paste viscosity, BDV breakdown viscosity, CPV cool paste viscosity, SBV setback viscosity, CSV consistency viscosity. b) Difference between Hwaseong and O. rufipogon. ns, *, and **indicate not significant, significant at P < 0.05 and P < 0.01, respectively. c) all the lines, d) 86 introgression possessing no O. rufipogon segments on the region of qDTH6, e) 13 introgression lines possessing the O. rufipogon segment on the region of qDTH6. f) ANOVA with 96 lines over 7 years. ns, *, and **indicate not significant, significant at P < 0.05 and P < 0.01, respectively. g) Numbers followed by the same letter in each row are not significantly different based on Duncan’s multiple range test at P < 0.05