Fig. 7

Genetic analysis and molecular mapping of ral1. a Frequency distribution of Al sensitivity and short root phenotype in ral1/WT F2 population. A total 148 F2 seedlings were exposed to –Al condition for 24 h and exposed to + Al (20 μM) condition for a further 24 h. Normal-root plants (Gray bar) and short-root plants (White bar) was determined based on that root elongation was more than 13 mm and less than 10 mm at the first 24 h, respectively. Relative root elongation (RRE) was used to evaluate their sensitivity to Al. b Molecular mapping of ral1. Bulked segregant analysis was used to determine the chromosome location of ral1 and a polymorphic marker Os06g004 boxed was found to be linked. Fifty F₂ plants with short roots and five additional markers were used to further map the gene. Left bar represents the chromosome 6 downloaded from RGP website (http://rgp.dna.affrc.go.jp). Genetic distance (cM) between neighboring makers and RGP genetic position (cM) were also indicated