Fig. 5

Genetic mapping of GL3b and GL6. a Recombinant plants possessing breakpoint at the Chr3 locus were screened from the F₃ population of segregation lines by using PCR markers RM7000 and BGLG_3-37.2_Hinf. We found four F₃ recombinant plants. Because the F₃ plants were heterozygous, we selected F₄ plants homozygous for BG23 or LG10 chromosome and recombinant chromosome to compare the phenotype for grain length. At least three homozygous plants were selected for each genotype. The grain length of these homozygous F₄ plants was measured by using 50 F₅ seeds. Two types of genotype selected among homozygous plants from each line are indicated to the left of the F₂ line number. The grain length of each line is indicated to the right of the F₂ line number. The results of the t-test for grain length between the plants with or without recombination are indicated to the right of the grain length of F₅ grains. GL3b gene locus was mapped into a 1.2-Mb region between BGLG_3-36.1_Hinf and BGLG_3-37.2_Hinf. b Recombinant plants possessing breakpoint at the Chr6L locus were screened from the F₃ population of segregation lines by using PCR markers BGLG_6-26.0_Hinf and BGLG_6-28.12_Hae. We found three F₃ recombinant plants. The grain length of homozygous F₄ plants was measured by using 50 F₅ seeds. GL6 gene locus was mapped into a 1.3-Mb region between BGLG_6-26.79_Hinf and BGLG_6-28.12_Hae. White and black boxes indicate BG23 and LG10 chromosomes, respectively. Student’s t-test was used to generate the P values. * indicates statistical significance from the neighboring value (P < 0.05). n.s. indicates no significance (P > 0.05). The numbers under the marker name indicate marker’s position (Mb)