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Rice

Table 1 Expression stability of candidate reference genes

From: Reference genes for accurate gene expression analyses across different tissues, developmental stages and genotypes in rice for drought tolerance

Classa1

Reference gene

Mean SD of ∆Ct

Rank on expression stability

in classb2

in totalc3

Single

LOC_Os03g13170

0.871

1

305

LOC_Os05g06770

0.929

2

336

LOC_Os08g03290

0.942

3

342

LOC_Os06g49480

1.073

4

366

LOC_Os08g19830

1.093

5

368

LOC_Os07g29390

1.159

6

373

LOC_Os11g06390

1.332

7

381

LOC_Os04g53620

1.561

8

382

LOC_Os02g06640

1.795

9

384

LOC_Os02g02890

1.862

10

385

2-gene

LOC_Os08g03290/LOC_Os08g19830

0.714

1

102

LOC_Os11g06390/LOC_Os08g03290

0.760

2

183

LOC_Os08g03290/LOC_Os05g06770

0.760

3

185

LOC_Os08g03290/LOC_Os03g13170

0.773

4

203

LOC_Os06g49480/LOC_Os08g03290

0.786

5

223

LOC_Os07g29390/LOC_Os05g06770

0.795

6

239

LOC_Os07g29390/LOC_Os03g13170

0.827

7

270

LOC_Os04g53620/LOC_Os08g19830

0.834

8

274

LOC_Os06g49480/LOC_Os02g06640

0.844

9

281

LOC_Os03g13170/LOC_Os08g19830

0.856

10

290

3-gene

LOC_Os07g29390/LOC_Os06g49480 /LOC_Os02g06640

0.684

1

49

LOC_Os06g49480/LOC_Os02g06640 /LOC_Os03g13170

0.686

2

54

LOC_Os08g03290/LOC_Os05g06770 /LOC_Os08g19830

0.697

3

71

LOC_Os02g02890/LOC_Os03g13170 /LOC_Os08g19830

0.698

4

75

LOC_Os06g49480/LOC_Os08g03290 /LOC_Os02g06640

0.699

5

77

LOC_Os06g49480/LOC_Os02g06640 /LOC_Os05g06770

0.702

6

80

LOC_Os07g29390/LOC_Os02g06640 /LOC_Os05g06770

0.705

7

86

LOC_Os02g06640/LOC_Os03g13170 /LOC_Os05g06770

0.708

8

88

LOC_Os02g02890/LOC_Os05g06770 /LOC_Os08g19830

0.708

9

89

LOC_Os08g03290/LOC_Os03g13170 /LOC_Os08g19830

0.708

10

91

4-gene

LOC_Os02g02890/LOC_Os08g03290 /LOC_Os05g06770/LOC_Os08g19830

0.618

1

1

LOC_Os02g02890/LOC_Os08g03290 /LOC_Os03g13170/LOC_Os08g19830

0.621

2

2

LOC_Os07g29390/LOC_Os06g49480 /LOC_Os02g06640/LOC_Os05g06770

0.622

3

3

LOC_Os11g06390/LOC_Os02g02890 /LOC_Os06g49480/LOC_Os08g03290

0.626

4

4

LOC_Os07g29390/LOC_Os06g49480 /LOC_Os02g06640/LOC_Os03g13170

0.627

5

5

LOC_Os02g02890/LOC_Os06g49480 /LOC_Os08g03290/LOC_Os08g19830

0.627

6

6

LOC_Os07g29390/LOC_Os02g06640 /LOC_Os03g13170/LOC_Os05g06770

0.630

7

7

LOC_Os11g06390/LOC_Os02g02890 /LOC_Os08g03290/LOC_Os03g13170

0.631

8

8

LOC_Os11g06390/LOC_Os02g02890 /LOC_Os08g03290/LOC_Os05g06770

0.634

9

9

LOC_Os07g29390/LOC_Os06g49480 /LOC_Os08g03290/LOC_Os02g06640

0.639

10

10

  1. a1 Category of reference genes
  2. b2 Rank in the same number of reference genes
  3. c3 Rank among all the reference genes tested
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