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Fig. 3 | Rice

Fig. 3

From: Development and validation of allele-specific SNP/indel markers for eight yield-enhancing genes using whole-genome sequencing strategy to increase yield potential of rice, Oryza sativa L.

Fig. 3

SCM2 marker. a Gene structure of APO1/SCM2. Three regions sequenced by the Sanger method were represented with primer pairs. b Agarose gel image analyzed by SCM2-indel1 marker from the donor line (Habataki), ST12, and 12 recipients. To separate two bands (117 bp and 105 bp) produced by the SCM2-indel1 marker, 4% agarose gel was used. c-d Application of SCM2-indel1 marker in the intermediate breeding line. Thirteen BC3F2 plants derived from Parao x Habataki cross were genotyped by the SCM2-indel1 marker and its PCR products were electrophoresed in 4 % agarose gel (c) and in Fragment Analyzer (d)

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