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Table 2 Annotation of water deficit, heat, and combined-stress-responsive proteins identified from spikelets of rice cultivar N22

From: Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Protein (spot)

pIa

Mra

Coverage (%)

Peptides matched

pIb

Mrb

Accession numberc

Putative group 3 pollen allergen (Spot 1)

5.55

06

67

79

5.37

12.0

Os06g0655200

Soluble inorganic pyrophosphatase (Spot 2)

6.11

36

91

187

5.90

23.0

Os10g0406100

Putative group 3 pollen allergen (Spot 3)

5.18

10

41

48

5.46

12.3

OSJNBa0050F15.8

Putative pollen allergen Ph1 p 11 (Spot 4)

5.49

30

63

111

5.76

19.0

Os06g0556600

Putative fructokinase (Spot 5)

5.12

56

73

248

5.02

35.0

P0498H04.29

17.4-kDa class I heat shock protein [HSP] (Spot 10)

5.74

20

57

93

5.80

17.9

OJ1364E02.6

16.9-kDa low-molecular-weight HSP (Spot 11)

6.15

19

84

127

6.18

16.9

P0443D08.5

Putative beta expansin (Spot 23)

6.54

44

54

145

6.34

28.6

OSJNBa0009C08.15

Unknown protein (Spot 7)

5.26

16

44

57

5.08

13.7

Os02g0800300

Unknown protein (Spot 19)

5.08

34

61

137

5.45

23.4

B1049E04.7-1

Novel proteind (Spot 25)

5.45

32

  1. Spots were excised from 2D gels where the spot was prominent irrespective of the treatment
  2. Coverage (%) percentage of sequence coverage obtained with identified peptides with Mascot software for the orthologous protein
  3. aExperimental pI, Mr
  4. bTheoretical pI and Mr
  5. cAccession number from NCBI (www.ncbi.nlm.nih.gov/gene/) all matching to rice
  6. dNot identified in the existing database