Table 4 Primer Sequences of SNAP Markers within Sorghum Genes
From: Molecular Markers for Sweet Sorghum Based on Microarray Expression Data
S. bicolor gene ID | Allele | WebSNAPER primer sequence | PCR product size (bp) | Allele presencea |
|---|---|---|---|---|
Sb01g043060 | T | F: GTAATATACTGACGCCAAAAGAGGCGGATT | 306 | BT |
R: TCAACTGCTGTTGTCGAGGACATTGG | ||||
A | F: TGTAATATACTGACGCCAAAAGAGGCGACTT | 307 | Ri-Top | |
R: TCAACTGCTGTTGTCGAGGACATTGG | ||||
Sb01g044810 | C | F: CAATCCTGCTCCCCAATCCAGACC | 334 | BT-Da-De-Sim |
R: GATTACGAGATCAGCGGTCTGGAAAGAAA | ||||
T | F: GCAATCCTGCTCCCCAATCCAGACT | 335 | Ri-He-IS-SC-M81 | |
R: GATTACGAGATCAGCGGTCTGGAAAGAAA | Top | |||
Sb02g000780 | A | F: TGGAGCAATACGAGGGCTACTCCAAA | 118 | BT |
R: AATCTTCAGAAACGCTCCATTTGTGCTG | ||||
G | F: TGGAGCAATACGAGGGCTACTCCATG | 118 | Ri-He-IS-SC-Da-De | |
R: AATCTTCAGAAACGCTCCATTTGTGCTG | M81-Top-Sim | |||
Sb02g006330 | G | F: TGTGGTACAGGTACACAAGCGAGAACATG | 115 | BT-IS-Da-De-M81 |
R: CCTTACAGGCATAACGAGTATGAGAGATTCATAACA | ||||
A | F: CTTATTTGTGGTACAGGTACACAAGCGAGAATAAA | 121 | Ri-Top-Sim | |
R: CCTTACAGGCATAACGAGTATGAGAGATTCATAACA | ||||
Sb03g012420 | C | F: GAAGCATTCTTTCCGATACAATATGGCCTATC | 164 | BT-He-SC-M81-Top |
R: TTCGATTAAAGGATTGTTGATGAAACTAGGGG | Sim | |||
T | F: GAAGCATTCTTTCCGATACAATATGGCCTACT | 164 | Ri-IS-Da | |
R: TTCGATTAAAGGATTGTTGATGAAACTAGGGG | ||||
Sb03g007840 | C | F: CCATAAATGTCATTGTGGAGACATCCGTTC | 161 | BT-He-IS-SC-M81 |
R: TGGAACGTCAAAACATTGACCGGAA | Top | |||
T | F: AAATGTCATTGTGGAGACATCCGGGT | 157 | Ri-Da-Sim | |
R: TGGAACGTCAAAACATTGACCGGAA | ||||
Sb03g027710 | T | F: GGTCATCGGTGATGGTGGAGAACCT | 343 | BT |
R: GGGAATTCGATTATGTCCATCACACCC | ||||
G | F: AGGTCATCGGTGATGGTGGAGATCTG | 344 | Ri-Da-Sim | |
R: GGGAATTCGATTATGTCCATCACACCC | ||||
Sb03g039090 | C | F: CGAACCCAACAACCTGTAACAATAAGCACTAC | 326 | BT-Da-De-Top-Sim |
R: GGAATTCGATTATCTCGGGGCTCATCTAC | ||||
A | F: GAACCCAACAACCTGTAACAATAAGCAGAAA | 325 | Ri-M81 | |
R: GGAATTCGATTATCTCGGGGCTCATCTAC | ||||
Sb04g0037170 | G | F: CACAAGCGACTTGAAACTGCGCTG | 131 | BT-IS-SC-Top |
R: GGCTTGACAACTGCTTCAACCTCTGC | ||||
C | F: CACAAGCGACTTGAAACTGCACCC | 131 | Ri-He-Da-De-M81 | |
R: GGCTTGACAACTGCTTCAACCTCTGC | Sim | |||
Sb07g005930 | T | F: CAGTTCTCCAATCCTTTCCTCTGTGGTCT | 146 | BT-He-SC-Da-M81 |
R: GTGAGAAGCGTGGGATGCTCATCAG | ||||
G | F: GTTCTCCAATCCTTTCCTCTGTGGTCG | 144 | Ri-IS-Top-Sim | |
R: GTGAGAAGCGTGGGATGCTCATCAG | ||||
Sb08g020760 | C | F: CAGAGGAAGCCCTTACACAGATCCGAC | 1,400 | BT-M81 |
R: TACCCACAGGTCTGGAAAGGGCAAG | ||||
T | F: CAGAGGAAGCCCTTACACAGATCCGAT | 416 | Ri-He-IS-SC-Top | |
R: TACCCACAGGTCTGGAAAGGGCAAG | Sim | |||
Sb08g008320 | T | F: GCAGTGGAAGGACATCATTGCCCAT | 174 | BT-He-Da-M81-Sim |
R: CTCTTCCGGGACGCGACGTTC | ||||
C | F: CAGTGGAAGGACATCATTGCCGTC | 173 | Ri-IS-SC-Top | |
R: CTCTTCCGGGACGCGACGTTC | ||||
Sb09g005280 | A | F: GCAGCACCGTCACCGGCACTA | 142 | BT |
R: GAGGCTCAATCAAGATCGTCTGCCC | ||||
G | F: CAGCACCGTCACCGGCATCG | 141 | Ri-He-IS-SC-Da-De | |
R: GAGGCTCAATCAAGATCGTCTGCCC | M81-Top-Sim | |||
Sb09g029170 | C | F: CTACTCTGAGATCATCAACGAGAGCGTGAAC | 124 | BT-He-SC-IS |
R: CCTAGATCCCAGGCGAGCCGTC | ||||
T | F: CTACTCTGAGATCATCAACGAGAGCGTGTTT | 124 | RI-Da-De-M81-Top | |
R: CCTAGATCCCAGGCGAGCCGTC | Sim | |||
Sb09g000820 | G | F: TCGAGAGCGATGCCTTCTGACATTG | ||
R: CCATATCTCCAGCCATCTTCAATGTTGTG | 128 | BT-Top | ||
A | F: CGAGAGCGATGCCTTCTGACAGCA | 130 | Ri | |
R: CCATATCTCCAGCCATCTTCAATGTTGTG | ||||
Sb09g006050 | C | F: ATAGAAGGCAGAATGAACGCTGGAAAGC | 105 | BT-Top |
R: GGGCAAGCAGGCCTGGAACTTC | ||||
A | F: AGAAGGCAGAATGAACGCTGGACTGA | 103 | Ri-He-IS-SC-Da-De | |
R: GGGCAAGCAGGCCTGGAACTTC | M81-Sim | |||
Sb10g007380 | T | F: GAACTACAGACATGCACAAGGATAGCAGGTT | 561 | BT-Top |
R: ATTGCATTCAGGAAGCTCGCTCGA | ||||
C | F: GAACTACAGACATGCACAAGGATAGCAGAGC | 561 | Ri-He-IS-SC-Da-De | |
R: ATTGCATTCAGGAAGCTCGCTCGA | M81 | |||
Sb10g002230 | G | F: CTTCAATCCGACAACCAAGTCGCTG | 197 | BT-He-IS-Top |
R: CTGGAACTGCAATGCGGCCATT | ||||
A | F: GCTTCAATCCGACAACCAAGTCGCTA | 197 | Ri-SC-Da-De-M81 | |
R: CTGGAACTGCAATGCGGCCATT | Sim |