Identified proteins | ||||||||
---|---|---|---|---|---|---|---|---|
Genotype | Growth stage, tissue | Treatment | Stress duration | Proteomic approach | T value | C value | Major result | References |
Drought stress | ||||||||
Nipponbare, Zhonghua (T) | Leaf sheath | Progressive water | 2 to 6Â days | 2D-PAGE/sequencing | 12 | 12 | Up-regulation of actin depolymerizing factor | [4] |
CT9993 (T), IR62266 | Leaf | Progressive water (23 days)/recovery (10 days) |  | 2D-PAGE/MALDI-TOF, Q-TOF MS | 42 | 16 | Stressed leaf WP = −2.4 Mpa/up-regulation of S-like RNase, actin depolymerizing factor and down-regulation of isoflavone reductase-like protein | [49] |
Salt stress | ||||||||
Nipponbare | Root (20Â days seedling) | 150Â mM NaCl | 0, 10, 24Â h | 2D-PAGE, Pro-Q diamond/MALDI-TOF MS | 214 | 28 | Pro-Q diamond was used to study phosphoproteome. About 214 proteins were detected as phosphoproteins | [8] |
IR651 (T) | Root (8Â weeks) | 100Â mM NaCl | 2Â weeks | 2D-PAGE/MALDI-TOF MS | 164 | 24 | Plasma membrane-associated protein was used. 1,4-Benzoquinone reductase, remorin and hypersensitive induced response protein were identified as salt responsive proteins | [42] |
Nipponbare, Pokkali (T) | Leaf sheath | 50, 100, 150Â mM NaCl | 6 to 48Â h | 2D-PAGE/Sequencing | 8 | 5 | IR36 was analyzed/coordinated response of different parts of the rice to NaCl | [1] |
Nipponbare | Root | 150 mM NaCl | 24, 48, 72 h | 2D-PAGE/MALDI-TOF MS | 54 | 12 | Several novel salt responsive proteins like α-NAC, COX6b-1, UGP | [57] |
Nipponbare | Leaf | 130Â mM NaCl | 4Â days | 2D-PAGE/LC MS/MS | 55 | 33 | Hydroponic culture system is best suited for proteomics of salt stress in rice seedling | [26] |
IR4630 (M) | Leaf lamina | 50Â mM NaCl | 1, 7Â days | 2D-PAGE/MALDI TOF-TOF MS | 32 | 11 | More responsive proteins in long- compare to short-term stress/defining threshold value to screen significant changes | [44] |
IR651 (T) | Young panicle | 70Â mM NaCl | 10Â days | 2D-PAGE/MALDI TOF-TOF MS | 13 | 13 | Constitutive high expression and/or up-regulation of ROS scavenging enzymes | [12] |
Cold stress | ||||||||
Doongara (S) | Anther/trinucleate stage | 12°C | 4 days | 2D-PAGE/MALDI-TOF MS | 70 | 18 | Chilling temperature stress at the young microspore stage enhances and induces partial degradation of proteins | [22] |
NA | Leaf | 15°C | 24 h | 2D-PAGE/MALDI-TOF, Q-TOF MS | 60 | 41 | 43% of identified proteins were located in chloroplast. So chloroplast proteome is virtually subjective to cold stress | [9] |
Doongara (S), HSC55 (T) | Anther/young microspore | 12°C | 1, 2, 4 days | 2D-PAGE/MALDI-TOF, Q-TOF MS, sequencing | 37 | 16 | The specific expression of responsive proteins tolerant compare to susceptible cultivar | [23] |
Nipponbare | Leaf | 6°C for 6, 24 h/24 h recovery | 2D-PAGE/MALDITOF-TOF MS | 96 | 85 | mRNA level of stress responsive genes was not correlated well with the protein level | [58] | |
Nipponbare, Kitaibuki (T), IR36, Er Jiu-Qing, Jamura, Kele (S) | Leaf | 1, 5, 15°C | 6 h | 32P-labeling, 2D-PAGE/sequencing | 50 | 10 | 32P-labeling was used to study phosphoproteome. Out of 10 cold responsible proteins, RuBisCO large-subunit was identified | [30] |
Nipponbare, Kitaake (T), Basmati 370 (S), transgenic (T) | Leaf sheath | 4°C | 24 h | 2D-PAGE/MALDI-TOF MS, sequencing | 456 | 6 | As transgenic rice, over-expression of CDPK13 and CRTintP1 was used. These two genes were binding to calreticulin | [34] |
Nipponbare | Basal part of leaf sheath | 4°C | 48 h | 2D-PAGE, lectin blot/MALDI-TOF MS | 250 | 22 | To analyze the glycosylation during cold stress, lectin blot was used | [33] |
Nipponbare | Leaf blades, leaf sheath, root | 4°C | 48 h | 2D-PAGE, RuBisCO removing/LC MS/MS | 400 | 39 | Antibody-affinity column was prepared to trap RuBisCO large subunit, and 4 proteins were newly detected after cold stress | [20] |
Dondjin | Leaf | 5, 10°C | 24, 72 h | 2D-PAGE, RuBisCO removing/MALDI-TOF MS, ESI MS/MS |  | 12 | Fractionation by PEG was used to trap RuBisCO large subunit | [37] |
Dondjin | Root | 10°C | 24, 72 h | 2D-PAGE/MALDI-TOF MS, ESI MS/MS | 600 | 27 | Acetyl transferase, phosphogluconate, NADP-specific isocitrate dehydrogenase, fructokinase, PrMC3, alpha-soluble N-ethylmaleinmide-sensitive factor attached protein and glyoxalase were found as cold responsive proteins | [38] |
Ozone stress | ||||||||
Nipponbare | Leaf | 0.2Â ppm Ozone | 24, 48, 72Â h | 2D-PAGE/Sequencing | 52 | 37 | Reduction in RuBisCO and induction of various defense/stress related proteins | [2] |
Koshihikari | Leaf | 5, 40, 80, 120Â ppb | 9Â days | 2D-PAGE/sequencing, MALDI-TOF MS | 300 | 20 | PR5, PR10 and RSPR10 were significantly induced after 2Â days exposure | [15] |
Other stresses | ||||||||
Hwayeong | Germinating seed | 0.2 to 1.5Â mM Copper | 4Â days | 2D-PAGE/MALDI TOF MS | 25 | 25 | The majority of these proteins were antioxidants or stress-related regulatory proteins | [3] |
Nipponbare, Zhonghua (T) | Basal part of leaf sheath | 0 to 1,000Â mM mannitol | 0 to 120Â h | 2D-PAGE/sequencing, MALDI-TOF MS | 327 | 15 | Heat shock protein and dnaK-type molecular chaperone were induced under osmotic, cold, salt, drought and ABA were reduced, whereas 26S proteasome regulatory subunit was found to be responsive only to osmotic stress | [59] |
Nipponbare | Leaf sheath | Water (wound) | 0, 12, 24, 48Â h | 2D-PAGE/Sequencing, MALDI-TOF MS | 29 | 12 | Identification of wound responsive proteins including Bowman-Birk trypsin inhibitor, receptor-like protein kinase, and calmodulin-related protein | [52] |
Multiple stresses | ||||||||
Nipponbare | leaf sheath, leaf blade, root | 50 mM NaCl (salt), 12°C (cold) | 24 h | 32P-labeling, 2D-PAGE/MALDI-TO, Q-TOF MS | 4, 4 | 2, 3 | Regulation of phosphorylation was studied | [24] |
Nipponbare | Root | 100 mM NaCl (salt), drought, 100 μM ABA | 15 h | 2D-PAGE/sequencing | 300 | 13 | RSPR10 was a novel rice PR10 protein, which was rapidly induced in roots by salt and drought stresses | [19] |